ABSTRACT This study aimed to determine the antibiotic profile of microorganisms isolated from urine samples of patients with community urine tract infections (UTI) admitted to the University Hospital of the Federal University of Sao Carlos to support an appropriate local empirical treatment. A retrospective cross-sectional study was conducted from October 2018 to October 2020. Data from 1,528 positive urine cultures for bacterial pathogens and antibiograms were tabulated. Bacterial species prevalence and their resistance profile were analyzed and compared by sex and age. For Gram-negative fermenting bacteria, resistance rates were compared between patients with previous hospitalization and the total of infections caused by this group. For comparisons, the Chi-square test was performed, using Fisher’s exact test when necessary (BioEstat program, adopting p ≤ 0.05). A multivariate analysis was applied to assess the effect of the studied variables in predicting multidrug resistance. Infections were more prevalent in women and older adults. Gram-negative bacteria represented 90.44% of total cultures. In both sexes, E. coli prevalence was significantly higher in adults compared with older adults (p < 0.0001). For several antibiotics, resistance rates were higher in the older adults compared with other ages and in patients with Gram-negative fermenting infections and previous hospitalization compared with the total of infections by this group of bacteria. The closer to the hospitalization, the higher the number of antibiotics with superior resistance rates. Resistance rates for aminoglycosides, carbapenems, ceftazidime, nitrofurantoin, piperacillin+tazobactam, and fosfomycin were less than 20%, considered adequate for empirical treatment. Only hospitalization in the previous 90 days was statistically significant in predicting infections by multidrug-resistant bacteria.

ABSTRACT Epidemiological, cross-sectional, descriptive, retrospective study of a quantitative nature, to trace the epidemiological and geospatial profile of the occurrences assisted by the Mobile Emergency Care Service. For that purpose, secondary data from the documents of the Mobile Emergency Care Service of a city in the interior of Rio Grande do Norte were used, considering the frequency of the variables: sex, age group, nature of occurrences, days of the week, ambulance sent, removal inter-hospital care and patient destination. Of the 363 occurrences, most were due to clinical causes, followed by traumatic, psychiatric, and gynecological/obstetric causes, with an emphasis on cardiovascular diseases and transport accidents. aged 19 to 59 years and over 60 years, with the Basic Support Unit providing the majority of care. Among the calls for clinical causes, women stood out, while for traumatic and psychiatric causes, men. The information contributes to the organization and planning of the service and the understanding of the morbidity profile. The geographic distribution highlights the need for integration between health agencies, for the institution of preventive actions, and the redirection of human and financial resources.

RESUMEN Estudio epidemiológico, transversal, descriptivo, retrospectivo, de carácter cuantitativo que tiene como objetivo trazar el perfil epidemiológico y geoespacial de los incidentes atendidos por el Servicio Móvil de Atención de Urgencias. Para ello se utilizan datos secundarios de dos documentos del Servicio Móvil de Atención de Emergencias de un municipio del interior de Rio Grande do Norte; considera la frecuencia de las variables: sexo, edad, naturaleza de los eventos, días de la semana, ambulancia enviada, retiro interhospitalario y destino del paciente. De los 363 sucesos, la mayoría se debió a causas clínicas, seguidas de causas traumáticas, psiquiátricas y ginecológicas/obstétricas, incluidas las enfermedades cardiovasculares y los accidentes de transporte. La frecuencia de ocurrencia fue ligeramente mayor en individuos masculinos con predominio de mujeres y edades entre los 19 y 59 años y mayores de 60 años; la Unidad Básica de Apoyo fue la responsable de la mayoría de los servicios. Entre los lesionados por causas clínicas destacan las mujeres, mientras que los hombres tienen causas traumáticas y psiquiátricas. Esta información contribuye a la organización y planificación de los servicios y a la comprensión del perfil de morbilidad. La distribución geográfica resalta la necesidad de integración entre las organizaciones de salud para implementar acciones preventivas y redireccionar recursos humanos y financieros.

Abstract Objective: to analyze the factors related to sleep disorders reported by Nursing professionals during the COVID-19 pandemic. Method: this is a cross-sectional and analytical study conducted with Nursing professionals from all Brazilian regions. Sociodemographic data, working conditions and questions about sleep disorders were collected. The Poisson regression model with repeated measures was used to estimate the Relative Risk. Results: 572 answers were analyzed, which revealed that non-ideal sleep duration, poor sleep quality and dreams about the work environment were predominant during the pandemic, with 75.2%, 67.1% and 66.8% respectively; as well as complaints of difficulty sleeping, daytime sleepiness and non-restorative sleep during the pandemic were reported by 523 (91.4%), 440 (76.9%) and 419 (73.2%) of the Nursing professionals, respectively. The relative risk of having such sleep disorders during the pandemic was significant for all variables and categories studied. Conclusion: non-ideal sleep duration, poor sleep quality, dreams about the work environment, complaints regarding difficulty sleeping, daytime sleepiness and non-restorative sleep were the predominant sleep disorders among Nursing professionals during the pandemic. Such findings point to possible consequences on health, as well as on the quality of the work performed.

Resumen Objetivo: analizar los factores relacionados con los trastornos del sueño que informaron los profesionales de enfermería durante la pandemia de COVID-19. Método: se trata de un estudio transversal y analítico realizado con profesionales de enfermería de todas las regiones de Brasil. Se recolectaron datos sobre caracterización sociodemográfica, condiciones de trabajo y preguntas sobre trastornos del sueño. Para estimar el Riesgo Relativo se utilizó el modelo de regresión de Poisson con medidas repetidas. Resultados: se analizaron 572 respuestas, que revelaron que durante la pandemia predominaron la duración del sueño no ideal, la mala calidad del sueño y los sueños sobre el ambiente laboral, con 75,2%, 67,1% y 66,8% respectivamente, además 523 (91,4%), 440 (76,9%) y 419 (73,2%) profesionales de enfermería manifestaron quejas de dificultad para conciliar el sueño, somnolencia diurna y sueño no reparador durante la pandemia, respectivamente. El riesgo relativo de padecer trastornos del sueño durante la pandemia fue significativo para todas las variables y categorías estudiadas. Conclusión: la duración del sueño no ideal, la mala calidad del sueño, los sueños sobre el ambiente laboral, las quejas de dificultad para conciliar el sueño, la somnolencia diurna y el sueño no reparador fueron los trastornos del sueño predominantes en los profesionales de enfermería durante la pandemia. Estos hallazgos indican posibles consecuencias para la salud, así como para la calidad del trabajo realizado.

Resumo Objetivo: analisar os fatores relacionados às alterações no sono relatadas pelos profissionais de enfermagem durante a pandemia de COVID-19. Método: trata-se de um estudo transversal e analítico, realizado com profissionais de enfermagem de todas as regiões do Brasil. Foram coletados dados de caracterização sociodemográfica, condições de trabalho e questões sobre alterações de sono. Para estimar o Risco Relativo foi utilizado o modelo de regressão de Poisson com medidas repetidas. Resultados: foram analisadas 572 respostas, as quais revelaram que a duração não ideal do sono, a má qualidade do sono e os sonhos com o ambiente de trabalho foram predominantes durante a pandemia, com 75,2%, 67,1% e 66,8% respectivamente, assim como as queixas de dificuldade ao dormir, sonolência diurna e sono não restaurador durante a pandemia foram relatadas por 523 (91,4%), 440 (76,9%) e 419 (73,2%) dos profissionais de enfermagem, respectivamente. O risco relativo de apresentar tais alterações de sono, durante a pandemia foi significativo para todas as variáveis e as categorias estudadas. Conclusão: duração não ideal do sono, má qualidade do sono, sonhos com o ambiente de trabalho, queixas de dificuldade ao dormir, sonolência diurna e sono não restaurador foram as alterações do sono predominantes entre os profissionais de enfermagem durante a pandemia. Estes achados apontam para possíveis consequências na saúde, bem como na qualidade do trabalho realizado.

Abstract Introduction: recent studies have suggested the use of neck circumference as a parameter capable of identifying risks of cardiometabolic complications and the accumulation of truncal fat caused by both antiretroviral therapy and the lifestyle of people with the human immunodeficiency virus (HIV). Objective: to investigate the relationship between neck circumference and anthropometric indicators and to assess cardiometabolic risk and truncal obesity through proposed cut-off points. Methods: cross-sectional study including 233 people with HIV. Demographic, socioeconomic, lifestyle and clinical data were collected using a structured questionnaire. The anthropometric evaluation included: weight, height, body mass index (BMI) measurements; waist (WC), neck (NC), arm and arm muscle circumferences; triceps and subscapular skinfolds and their sum. ROC curves were constructed to determine the accuracy of NC in predicting cardiometabolic risk in people living with HIV. Results: the sample was 57.5 % male, with a mean age of 38.4 years (95 % CI: 37.2-39.7 years). NC showed a positive and significant correlation with all anthropometric variables analyzed (p < 0.05), and a higher correlation strength with WC and BMI. The NC cut-off point selected as a predictor of risk of cardiac metabolic complications and truncal obesity in women was ≥ 32.4 cm, considering both WC and BMI. For men, the NC cut-off points were different when considering WC (≥ 39.6 cm) and BMI (≥ 38.1 cm) as a reference. It is worth noting that NC performed well in ROC curve analysis for men, while in women it was a poor performance. Conclusion: NC proved to be a promising indicator in the assessment of nutrition and health of people living with HIV, especially in men.

Resumen Introducción: estudios recientes han sugerido el uso de la circunferencia del cuello como parámetro capaz de identificar los riesgos de complicaciones cardiometabólicas y la acumulación de grasa troncal causados tanto por la terapia antirretroviral como por el estilo de vida de las personas con el virus de la inmunodeficiencia humana (VIH). Objetivo: investigar la relación entre la circunferencia del cuello y los indicadores antropométricos y evaluar el riesgo cardiometabólico y la obesidad troncal a través de los puntos de corte propuestos. Métodos: estudio transversal que incluyó a 233 personas con VIH. Se recogieron datos demográficos, socioeconómicos, de estilo de vida y clínicos mediante un cuestionario estructurado. La evaluación antropométrica incluyó: medidas de peso, altura, índice de masa corporal (IMC); circunferencias de cintura (CC), cuello (CN), brazo (CA) y músculo del brazo (MCB); pliegues cutáneos del tríceps y subescapular y su suma. Se construyeron curvas ROC para determinar la precisión de la CN en la predicción del riesgo cardiometabólico en personas que viven con el VIH. Resultados: el 57,5 % de la muestra eran varones, con una edad media de 38,4 años (IC 95 %: 37,2-39,7 años). La CN mostró una correlación positiva y significativa (p < 0,05) con todas las variables antropométricas analizadas, y una mayor fuerza de correlación con la CC y el IMC. El punto de corte de la CN seleccionado como predictor de riesgo de complicaciones metabólicas cardiacas y obesidad troncular en mujeres fue ≥ 32,4 cm, considerando tanto la CC como el IMC. En el caso de los hombres, los puntos de corte de la CN fueron diferentes al considerar como referencia la CC (≥ 39,6 cm) y el IMC (≥ 38,1 cm). Cabe destacar que la CN obtuvo buenos resultados en el análisis de la curva ROC en el caso de los hombres, mientras que en el de las mujeres fue deficiente. Conclusión: la CN demostró ser un indicador prometedor en la evaluación de la nutrición y la salud de las personas que viven con el VIH, especialmente en los hombres.

ABSTRACT Patients undergoing hematopoietic stem cell transplantation (HSCT) might present acute and late toxicities and the oral tissues are frequently affected. With the survival increasing, patients show late and long-term morbidities, and there is an important association between the general and the oral health. The first and second parts of this Consensus have showed the importance of the adequacy of oral health in the pre-HSCT, and the main alterations and oral care during the period of admission for HSCT. This third part aims to review specific themes of post-HSCT dental care, such as graft-versus-host disease (GVHD) and the pediatric patient. It also aims to review pertinent subjects, both during the HSCT period and post-HSCT, concerning quality of life, pain, cost-effectiveness, and remote care. Based on this review, it is evident the importance of the work of the dental surgeon (DS) in the follow-up and treatment of the HSCT patient, always collaborating with the whole multidisciplinary team.

ABSTRACT The oral involvement in the Hematopoietic Stem Cell Transplantation is well described in the literature. The goal of the dental treatment and management of the oral lesions related to the HSCT is to reduce the harm caused by preexisting oral infection or even the worsening of oral acute/chronic GVHD and late effects. The aim of this guideline was to discuss the dental management of patients subjected to HSCT, considering three phases of the HSCT: pre-HSCT, acute phase, and late phase. The literature published from 2010 to 2020 was reviewed in order to identify dental interventions in this patient population. The selected papers were divided into three groups: pre-HSCT, acute and late, and were reviewed by the SBTMO Dental Committee's members. When necessary, an expertise opinion was considered for better translating the guideline recommendations to our population dental characteristics. This manuscript focused on the pre-HSCT dental management. The objective of the pre-HSCT dental management is to identify possible dental situations that On behalf of the Dental Committee of the Brazilian Society of Gene Therapy and Bone Marrow Transplantation (SBTMO) can worsening during the acute phase after the HSCT. Each guideline recommendations were made considering the Dentistry Specialties. The clinical consensus on dental management prior to HSCT provides professional health caregivers with clinical setting-specific information to help with the management of dental problems in patients to be subjected to HSCT.

The general objective of this research was to analyze and understand the teacher's perception of violence within sports practice during Physical Education classes at Colégio Pedro II. This is a case study research of a qualitative nature. The research was conducted with Physical Education teachers from different units of the school. The documental analysis of the Institutional Pedagogical Political Project and the teachers' planning, and the semi-structured interview were used as data collection instruments. Data analysis used categorization as a methodological procedure. The main expressions of violence found were exclusion and self-exclusion from practical activities; gender violence; and verbal violence. Society and media sports were identified as influencing elements in the reproduction of behaviors. Sports content and soccer were identified as practices with a greater propensity to situations of violence. The Physical Education analyzed here aims at citizen formation, the recognition of cultural diversity and respect for differences, valuing all identities. Despite the observation of the work with violence in its interfaces, we highlight the absence of an intentional work for the theme of violence in sports specifically.  We reiterate the importance of such work in order to assist in the awareness and use of strategies to prevent violent conduct within the sports field in the school environment.  

El objetivo general de esta investigación fue analizar y comprender la percepción del profesor sobre la violencia dentro de la práctica deportiva durante las clases de Educación Física en el Colegio Pedro II. Esta es una investigación de estudio de caso de naturaleza cualitativa. La investigación se realizó con profesores de Educación Física de diferentes unidades de la escuela. El análisis documental del Proyecto Político Pedagógico Institucional y la planificación docente, y la entrevista semiestructurada fueron utilizados como instrumentos de recolección de datos. El análisis de los datos utilizó la categorización como procedimiento metodológico. Las principales expresiones de violencia encontradas fueron la exclusión y la autoexclusión de las actividades prácticas; violencia de género; y violencia verbal. La sociedad y los deportes mediáticos fueron identificados como elementos influyentes en la reproducción de comportamientos. Los contenidos deportivos y el fútbol fueron identificados como prácticas con mayor propensión a situaciones de violencia. La Educación Física aquí analizada tiene como objetivo la formación ciudadana, el reconocimiento de la diversidad cultural y el respeto a las diferencias, valorando todas las identidades. A pesar de la observación del trabajo con violencia en sus interfaces, destacamos la ausencia de un trabajo intencional para el tema de la violencia en el deporte específicamente.  Reiteramos la importancia de este trabajo para ayudar en la concientización y el uso de estrategias para prevenir conductas violentas dentro del campo deportivo en el entorno escolar.

O objetivo geral dessa pesquisa foi analisar e compreender a percepção docente a respeito da violência nos esportes durante as aulas de Educação Física no Colégio Pedro II. Trata-se de uma pesquisa de estudo de caso, de natureza qualitativa. A pesquisa foi realizada com docentes de Educação Física de diferentes unidades do Colégio. Foram utilizados como instrumentos de coleta de dados a análise documental do Projeto Político Pedagógico Institucional e dos planejamentos docentes, e a entrevista semiestruturada. A análise de dados utilizou a categorização como procedimento metodológico. As principais expressões de violência encontradas foram a exclusão e autoexclusão das atividades práticas; a violência de gênero; e a violência verbal. A sociedade e os esportes midiáticos foram identificados como elementos influenciadores na reprodução de comportamentos. Já o conteúdo esportivo e o futebol foram apontados como práticas com maior propensão às situações de violência. A Educação Física aqui analisada objetiva a formação cidadã, o reconhecimento da diversidade cultural e o respeito às diferenças, valorizando todas as identidades. Apesar da constatação do trabalho com a violência nas suas interfaces, destacamos a ausência de um trabalho intencional para a temática da violência nos esportes especificamente.  Reiteramos a importância de tal trabalho a fim de auxiliar na conscientização e no uso de estratégias de prevenção de condutas violentas dentro do campo desportivo no ambiente escolar.

Abstract Objective: The objective of this study was to analyze masticatory characteristics in children from 7 to 10 years, and to compare these characteristics among normal weight, overweight and obese children. Methods: This is a cross-sectional study, conducted in northeast Brazil, with a sample of 160 children, aged 7 to 10 years. The authors evaluated: nutritional and dental status; food intake; masticatory parameters and orofacial myofunctional characteristics. The children were divided according to nutritional status into normal weight (n = 101), overweight (n = 33) and obesity (n = 26) groups. Results: The results showed that Obese children had a larger bite size (g) (median = 6.0, Q1-Q3 = 4.0-9.0, p = 0.049) and performed fewer masticatory sequences (median = 3.0, Q1-Q3 = 2.0-3.2, p = 0.024) than children with normal weight (median = 5.0, Q1-Q3 = 4.0-7.0; median = 4.0, Q1-Q3 = 3.0-5.0). Furthermore, obese children finished feeding in a shorter time (s) (median = 62.5, Q1-Q3 = 50.5-70.0, p = 0.039) than normal weight children (median = 66.0, Q1-Q3 = 56.5-78.0) and overweight children (median = 66.0, Q1-Q3 = 58.0-81.5). Conclusions: The present results suggest that obese children present changes in mastication, evidenced by larger bite-size, performing fewer masticatory sequences and rapid mastication, which may contribute to increased food consumption and the development of excess weight.

Abstract Objective: The aim of our study was to evaluate the expression of MMP-2 and MMP-9 as a prognostic factor in patients diagnosed with Hodgkin Lymphoma (HL). Methods: In the present study, 45 paraffin biopsies from patients up to 19 years old diagnosed with HL were used in two referral hospitals in the state of Pernambuco, Brazil. Risk groups were classified into favorable and unfavorable, according to Ann Arbor. The expression of matrix metalloproteinases 2 and 9 and their inhibitors was performed by immunohistochemistry (IHC). Data were analyzed using the GraphPad Prism 5 program. Results: MMP-2 intensity pattern was stronger (> 10% of the total field) in patients with stage III/ IV and B symptoms. MMP-2 showed an association with the risk group (p = 0.0388). That is, the stronger the MMP-2 marking, the greater the unfavorable risk. However, for MMP-9 there was no difference in the stronger intensity pattern in relation to stages I/II and III/IV, only in the presence of B symptoms. MMP-9 showed an association with B Symptoms (p = 0.0411). Therefore, patients with B symptoms have higher MMP-9 expression. Conclusion: Our results suggest that MMP-2 expression is associated with HL progression. While MMP-9 expression is related to the clinical worsening of these patients. However, further studies are needed to evaluate the exact role of these proteins in hematologic malignancies.

Resumo Objetivo: analisar o consumo de marcadores de alimentação saudável entre adolescentes escolares de Pelotas, Rio Grande do Sul, Brasil, em 2019. Métodos: estudo transversal, realizado nas escolas vinculadas ao Programa Saúde na Escola; o consumo dos cinco marcadores (feijão, leite, frutas, vegetais cozidos, saladas cruas) em até cinco dias antecedentes ao estudo, obtido mediante questionário autoaplicado, foi definido como regular; a associação de variáveis demográficas, socioeconômicas e comportamentais com os desfechos foi analisada por regressão de Poisson. Resultados: entre os 797 entrevistados, 2,8% (IC95% 1,8;4,1) referiram consumo regular; o feijão foi o alimento mais consumido regularmente (48,3%); frutas, vegetais cozidos e saladas cruas foram consumidos regularmente por menos de 30% dos escolares; praticar atividade física, tomar café da manhã e realizar refeições com a família estiveram associados ao consumo regular dos alimentos avaliados. Conclusão: o consumo regular de alimentos saudáveis, encontrado baixo nessa população, associou-se com fatores comportamentais.

Resumen Objetivo: analizar el consumo de marcadores de alimentación saludable entre estudiantes adolescentes de Pelotas, Rio Grande do Sul, Brasil, en 2019. Métodos: estudio transversal realizado en escuelas vinculadas al Programa Salud en la Escuela. Se definió como regular el consumo de los cinco marcadores (porotos, leche, frutas, vegetales cocidos y ensaladas crudas) dentro de los cinco días previos al estudio, obtenidos a través de un cuestionario autoadministrado. La asociación entre variables demográficas, socioeconómicas y conductuales con los resultados se analizó mediante la regresión de Poisson. Resultados: entre los 797 encuestados, el 2,8% (IC95% 1,8; 4,1) informó un consumo regular. Los porotos fueron el alimento más consumido (48,3%). Frutas, verduras cocidas y ensaladas crudas fueron consumidas regularmente por menos del 30% de los estudiantes. La práctica de actividad física, el desayuno y las comidas en familia se asociaron con mayor consumo de los alimentos evaluados. Conclusión: el consumo regular de alimentos saludables fue bajo en esta población y se asoció con factores conductuales.

Abstract Objective: to analyze the consumption of healthy eating markers among adolescent students in Pelotas, Rio Grande do Sul, Brazil, in 2019. Methods: this was a cross-sectional study carried out in schools taking part in the Health at School Program. Consumption of the five markers (beans, milk, fruit, cooked vegetables and raw salads) within five days prior to the study, obtained through a self-administered questionnaire, was defined as regular. Association between demographic, socioeconomic and behavioral variables and the outcomes was analyzed using Poisson regression. Results: among the 797 respondents, 2.8% (95%CI 1.8;4.1) reported regular consumption. Beans were the most regularly consumed food (48.3%); fruit, cooked vegetables and raw salads were consumed regularly by less than 30% of the students. Doing physical activities, having breakfast and having meals with the family were associated with regular consumption of the foods assessed. Conclusion: regular consumption of healthy foods was low in this population, and was associated with behavioral factors.

A formação médica é considerada estressante devido a fatores como: extensa carga horária, atividades práticas complexas, muito conteúdo e alta exigência de dedicação. Programas de mentoria podem amenizar esse estresse acadêmico. Com o objetivo de investigar a percepção dos estudantes de um programa de mentoria sobre o estresse da formação médica e a mentoria como recurso de enfrentamento, desenhou-se um estudo do tipo transversal com metodologia qualitativa. Selecionaram-se 12 alunos e realizaram-se sessões de grupo focal. Os dados produzidos foram submetidos à análise temática, resultando em duas categorias de estressores – 1. ser estudante e 2. ambiente acadêmico – e em duas categorias de elementos protetores – 1. autocuidado e 2. mentoria. A mentoria foi considerada recurso de enfrentamento por ser um espaço de reflexão e desenvolvimento de habilidades e comportamentos que aumentam competências relacionais, além de propiciar acolhimento, alívio psicológico, pertencimento e efeito tranquilizador.

La formación médica se considera estresante debido a factores tales como: extensa carga horaria, actividades prácticas complejas, mucho contenido, alta exigencia de dedicación. Programas de tutoría pueden amenizar ese estrés académico. Con el objetivo de investigar la percepción de los estudiantes de un programa de tutoría sobre el estrés de la formación médica y la tutoría como recursos de enfrentamiento, se diseñó un estudio del tipo transversal con metodología cualitativa. Se seleccionaron 12 alumnos y se realizaron sesiones de grupo focal. Los datos producidos se sometieron a análisis temático, resultando en dos categorías de factores de estrés: 1. ser estudiante, y 2. ambiente académico; y dos categorías de elementos protectores: 1. autocuidado, y 2. tutoría. La tutoría se consideró recurso de enfrentamiento por ser espacio de reflexión y desarrollo de habilidades y comportamientos que aumentan competencias relacionales además de propiciar amparo, alivio psicológico, pertenencia y efecto tranquilizador.

Medical training is considered stressful due to factors such as heavy course load, complex practical activities, the large volume of content and high expectations in terms of dedication. Mentoring programs can alleviate academic stress. The aim of this qualitative cross-sectional study was to investigate medical students’ perceptions of a mentoring program designed to reduce stress and mentoring as a tool for coping. We selected 12 students and held focus group sessions. We performed a thematic analysis, which resulted in the identification of two categories of stressors and protectors: being a student and the academic environment and self-care and mentoring, respectively. Mentoring was considered a coping resource because it provided a space for reflection and helped students develop skills and behavior that enhance relational skills, in addition to providing a welcoming environment, psychological relief, a feeling of belonging and a tranquilizing effect.

Abstract Acinetobacter spp. are one of the main pathogens responsible for healthcare-associated infections and are associated with high rates of morbidity and mortality globally, mainly because of their high capacity to present and develop resistance to antimicrobials. To identify species of the Acinetobacter and their resistance profiles from samples collected from hospitalized patients, health professionals and hospital environmental sources in the intensive care units of different public reference hospitals in Porto Velho City, Rondônia, Western Brazilian Amazon. Isolates were identified using microbiological and molecular techniques. The antimicrobial susceptibility profile was determined by disk diffusion. A total of 201 Acinetobacter spp. isolates were identified, of which 47.3% originated from hospital structures, 46.8% from patients and 6% from healthcare professionals. A. baumannii and A. nosocomialis were the most prevalent, with frequency of 58.7% and 31.8%, respectively. Regarding the susceptibility profile, it was observed that 56.3% were classified as multidrug-resistant and 76.2% of the samples belonging to A. baumannii were resistant to carbapenems. In contrast, 96.9% were susceptible to polymyxin B and 91.3% to doxycycline. The data presented here can be used to guide and strengthen the control of multidrug-resistant infections caused by Acinetobacter spp., in addition to improving providing information from a traditionally unassisted region of Brazil.

ABSTRACT BACKGROUND: Social distancing has led to lifestyle changes among older adults during the coronavirus disease 2019 (COVID-19) pandemic. OBJECTIVES: This study aimed to estimate the prevalence risk of sarcopenia (RS) and investigate its associated factors during the COVID-19 pandemic in older Brazilian adults. DESIGN AND SETTING: Cross-sectional observational analysis of baseline data as part of the Remobilize Study. METHODS: Participants in the study were older adults (≥ 60 years), excluding those who were bedridden or institutionalized. The data collected consisted of answers about the RS (SARC-F), functional status, walking, sedentary behavior (SB), pain, comorbidity, and life space mobility. RESULTS: A total of 1,482 older adults (70 ± 8.14 years, 74% women) participated in the study, and an RS prevalence of 17.1% was found. (95% confidence interval [CI] 15.25–19.15%). The adjusted multivariate model showed a significant association between RS and functional limitation (odds ratio [OR]: 19.05; CI 13.00–28.32), comorbidity (OR: 5.11; CI 3.44–7.81), pain (OR: 4.56; CI 3.33–6.28), total walking (OR: 0.99; CI 0.99–1.00), SB of 8–10 hours (OR: 1.85; CI 1.15–2.93), and SB of > 10 hours (OR: 3.93; CI 2.48–6.22). RS was associated with mobility during the pandemic (OR: 0.97; CI 0.96–0.98). P < 0.05. CONCLUSIONS: During the pandemic, the prevalence of RS in older Brazilians was estimated at 17.1%. Moderate to severe functional limitation, comorbidities, presence of pain, walking, longer SB period, and reduced life space mobility significantly contributed to RS in older adults during the pandemic.

Abstract Objective: Given the high proliferative activity of germinal matrix and its direct correlation with hypoxemia, it is necessary to investigate the possible molecular regulation pathways, to understand the existing clinical relationship between the hypoxic-ischemic insult and the biomarkers NF-kB, AKT-3, Parkin, TRK-C and VEGFR-1. Methods: A hundred and eighteen germinal matrix samples of the central nervous system of patients who died in the first 28 days of life were submitted to histological and immunohistochemistry analysis to identify the tissue immunoexpression of those biomarkers related to asphyxia, prematurity, and death events within 24h. Results: A significantly increased tissue immunoexpression of NF-kB, AKT-3 and Parkin was observed in the germinal matrix of preterm infants. In addition, significantly decreased tissue immunoexpression of VEGFR-1 and NF-kB was observed in patients who experienced asphyxia followed by death within 24 hours. Conclusions: The results suggest a direct involvement between the hypoxic-ischemic insult and NF-kB and VEGFR-1 markers since a decreased immunoexpression of these biomarkers was observed in asphyxiated patients. Furthermore, it is suggested that there was not enough time for VEGFR-1 to be transcribed, translated and expressed on the surface of the plasma membrane. This temporality can be observed in the relationship between NF-kB expression and the survival time of individuals who died within 24 hours, suggesting that this factor is essential for the production of VEGFR-1 and, therefore, to carry out the necessary remodeling effect to neovascularize the affected region.

RESUMO Objetivo: Dada a alta atividade proliferativa da matriz germinativa e sua correlação direta com a hipoxemia, é necessário investigar as possíveis vias de regulação molecular para entender a relação clínica existente entre o insulto hipóxico-isquêmico e os biomarcadores NF-kB, AKT -3, Parkina, TRK-C e VEGFR-1. Métodos: Cento e dezoito amostras de matriz germinativa do sistema nervoso central de pacientes que faleceram nos primeiros 28 dias de vida foram submetidas a análise histológica e imuno-histoquímica para identificar a imunoexpressão tecidual desses biomarcadores relacionados a eventos de asfixia, prematuridade e óbito em 24 horas. Resultados: Observou-se uma imunoexpressão tecidual significativamente aumentada de NF-kB, AKT-3 e Parkin na matriz germinativa de prematuros. Além disso, constatou-se uma imunoexpressão tecidual significativamente diminuída de VEGFR-1 e de NF-kB em pacientes que apresentaram asfixia seguida de morte em 24 horas. Conclusões: Os resultados sugerem o envolvimento direto entre o insulto hipóxico-isquêmico e os marcadores NF-kB e VEGFR-1, visto que se observou uma imunoexpressão diminuída destes biomarcadores nos pacientes asfixiados. Além disso, sugere-se que não houve tempo suficiente para que o VEGFR-1 fosse transcrito, traduzido e expresso na superfície da membrana plasmática. Essa temporalidade pode ser observada na relação entre a expressão de NF-kB e o tempo de vida dos indivíduos que morreram em 24 horas, o que sugere que esse fator é essencial para a produção do VEGFR-1 e, portanto, para realizar o efeito remodelador necessário para neovascularizar a região afetada.

ABSTRACT Objectives to analyze and compare the level of knowledge about pressure ulcer prevention among nurses and nursing technicians who work in Intensive Care Units and nursing students in the last year of the course. Method a cross-sectional, descriptive-exploratory study with a quantitative approach, using the Pieper’s Pressure Ulcer Knowledge Test. Data were analyzed using the Statistical Package for Social Science, using descriptive and inferential statistics (ANOVA-One Way, Shapiro-Wilk normality test and assumption of homogeneity using Levene’s test). Results the research sample included 70 participants, being 22 nurses, 14 nursing technicians and 34 undergraduate nursing students. It was observed that only 22.7% of nurses, 7.1% of technicians and 0.0% of students reached the percentage of correct answers ≥ 90% recommended by the knowledge test. Conclusion and implications for practice the results found in this study made it possible to identify an insufficient level of knowledge and a mistaken perception of aptitude, related to the pressure ulcer prevention, among the study participants, with emphasis on technicians and undergraduates in nursing. Therefore, this research points to the need for educational actions aimed at training the aforementioned public.

RESUMEN Objetivos analizar y comparar el nivel de conocimiento sobre prevención de úlceras por presión entre enfermeros y técnicos de enfermería que actúan en Unidades de Cuidados Intensivos y estudiantes de enfermería en el último año del curso. Método estudio transversal, descriptivo-exploratorio y de abordaje cuantitativo, utilizando el Pieper’s Pressure Ulcer Knowledge Test. Los datos fueron analizados mediante el Statistical Package for Social Science, utilizando estadística descriptiva e inferencial (ANOVA-One Way, prueba de normalidad de Shapiro-Wilk y suposición de homogeneidad mediante la prueba de Levene). Resultados la muestra contó con 70 participantes, siendo 22 enfermeros, 14 técnicos de enfermería y 34 estudiantes de graduación en enfermería. Se observó que sólo el 22,7% de los enfermeros, el 7,1% de los técnicos y el 0,0% de los estudiantes alcanzaron el porcentaje de aciertos ≥ 90% recomendado por la prueba de conocimiento. Conclusión e implicaciones para la práctica los resultados encontrados permitieron identificar un nivel de conocimiento insuficiente y una percepción de aptitud errónea, relacionada con la prevención de úlceras por presión, entre los participantes del estudio, con énfasis en los técnicos y estudiantes de enfermería. Por lo tanto, esta investigación apunta la necesidad de acciones educativas dirigidas a la formación de dicho público.

RESUMO Objetivos analisar e comparar o nível de conhecimento sobre prevenção de lesão por pressão entre enfermeiros e técnicos de enfermagem que atuam em Unidades de Terapia Intensiva e graduandos em enfermagem no último ano do curso. Método estudo transversal, de caráter descritivo-exploratório e de abordagem quantitativa, utilizando o Pieper’s Pressure Ulcer Knowledge Test. Os dados foram analisados através do Statistical Package for Social Science, mediante estatística descritiva e inferencial (ANOVA-One Way, teste de normalidade de Shapiro-Wilk e pressuposto de homogeneidade através do teste de Levene). Resultados a amostra da pesquisa abarcou 70 participantes, sendo 22 enfermeiros, 14 técnicos de enfermagem e 34 graduandos em enfermagem. Foi visto que apenas 22,7% dos enfermeiros, 7,1% dos técnicos e 0,0% dos graduandos em enfermagem atingiram o percentual de acertos ≥ 90% recomendado pelo teste de conhecimento. Conclusão e implicações para a prática os resultados encontrados neste estudo permitiram identificar um nível insuficiente de conhecimento e uma percepção de aptidão equivocada, relacionados à prevenção de lesão por pressão, entre os participantes do estudo, com ênfase para os técnicos e para os graduandos em enfermagem. Logo, esta pesquisa aponta para a necessidade de ações educativas que visem a capacitação do público supramencionado.